NDUFS5 Polyklonaler Antikörper

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• KD/KO Validated

NDUFS5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15224-1-PBS

Synonyme

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  WB analysis using 15224-1-AP (same clone as 15224-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15224-1-AP (NDUFS5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15224-1-AP (same clone as 15224-1-PBS)

  WB result of NDUFS5 antibody (15224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS5 transfected HEK-293 cells. This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15224-1-AP (same clone as 15224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15224-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15224-1-PBS bindet in WB, IHC, Indirect ELISA NDUFS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFS5 fusion protein Ag7441
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC001884
• Gene symbol: NDUFS5
• Gene ID (NCBI): 4725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150).