NDUFS5 Monoklonaler Antikörper

NDUFS5 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C2A5

Kat-Nr. : 66053-1-PBS

Synonyme

2C2A5, CI 15 kDa, CI-15 kDa, Complex I 15 kDa, Complex I-15 kDa

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  WB analysis of HepG2 using 66053-1-Ig (same clone as 66053-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66053-1-Ig (same clone as 66053-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66053-1-Ig (same clone as 66053-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66053-1-Ig (same clone as 66053-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66053-1-Ig (same clone as 66053-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50607-1

  Cytometric bead array standard curve of MP50607-1, NDUFS5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66053-2-PBS. Detection antibody: 66053-1-PBS. Standard:Ag7663. Range: 3.125-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66053-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA NDUFS5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NDUFS5 fusion protein Ag7663
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC001884
• Gene symbol: NDUFS5
• Gene ID (NCBI): 4725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach's NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.