NDUFS6 Monoklonaler Antikörper

NDUFS6 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F4H3

Kat-Nr. : 68329-1-PBS

Synonyme

2F4H3, CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A

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  WB analysis of rabbit heart using 68329-1-Ig (same clone as 68329-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (68329-1-Ig, Clone: 2F4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68329-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NDUFS6 und zeigt Reaktivität mit Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NDUFS6 fusion protein Ag6286
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 13-15 kDa
• GenBank-Zugangsnummer: BC046155
• Gene symbol: NDUFS6
• Gene ID (NCBI): 4726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.