NDUFS7 Polyklonaler Antikörper

NDUFS7 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15728-1-PBS

Synonyme

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  WB analysis of A549 using 15728-1-AP (same clone as 15728-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15728-1-AP (NDUFS7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 15728-1-AP (same clone as 15728-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 15728-1-AP (NDUFS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15728-1-AP (same clone as 15728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15728-1-AP (NDUFS7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15728-1-AP (same clone as 15728-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDUFS7 antibody (15728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15728-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15728-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NDUFS7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFS7 fusion protein Ag8471
• Vollständiger Name: NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase)
• Berechnetes Molekulargewicht: 213 aa, 24 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC005954
• Gene symbol: NDUFS7
• Gene ID (NCBI): 374291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFS7(NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial) protein is one of the most conserved subunits of mitochondrial respiratory chain complex I and plays a central role in the interaction with the electron acceptor ubiquinone and in the proton-translocating mechanism(PMID:15269216).Defects in NDUFS7 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).