NEDD1 Polyklonaler Antikörper

NEDD1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13993-1-PBS

Synonyme

---

• 

  WB analysis using 13993-1-AP (same clone as 13993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 13993-1-AP (same clone as 13993-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 13993-1-AP (same clone as 13993-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 13993-1-AP (same clone as 13993-1-PBS)

  IP result of anti-NEDD1 (IP:13993-1-AP, 3ug; Detection:13993-1-AP 1:800) with HeLa cells lysate 2150ug. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

13993-1-PBS bindet in WB, IHC, IP, Indirect ELISA NEDD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEDD1 fusion protein Ag5077
• Vollständiger Name: neural precursor cell expressed, developmentally down-regulated 1
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 75 kDa, 62 kDa
• GenBank-Zugangsnummer: BC027605
• Gene symbol: NEDD1
• Gene ID (NCBI): 121441
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEDD1 is required for mitosis progression. NEDD1 promotes the nucleation of microtubules from the spindle. This antibody works well in WB, IHC, IP and IF application.