NEDD4 Rekombinanter Antikörper

NEDD4 Rekombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

230478F1

Kat-Nr. : 83112-1-RR

Synonyme

KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein

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  WB analysis using 83112-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83112-1-RR (NEDD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 83112-1-RR

  IP result of anti-NEDD4 (IP:83112-1-RR, 4ug; Detection:83112-1-RR 1:1000) with NIH/3T3 cells lysate 1505 ug.

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  IF Staining of U2OS using 83112-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-1-RR, Clone: 230478F1 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83112-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83112-1-RR against Human NEDD4 were performed. The affinity constant is 14.8 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83112-1-RR bindet in WB, IF/ICC, IP, ELISA NEDD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NEDD4 fusion protein Ag16291
• Vollständiger Name: neural precursor cell expressed, developmentally down-regulated 4
• Berechnetes Molekulargewicht: 1319 aa, 149 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC152452
• Gene symbol: NEDD4
• Gene ID (NCBI): 4734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).