NEDD4 Rekombinanter Antikörper

NEDD4 Rekombinant Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230478F1

Kat-Nr. : CL750-83112

Synonyme

230478F1, Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4

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  WB analysis using CL750-83112

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83112 (NEDD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-83112 bindet in WB NEDD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NEDD4 fusion protein Ag16291
• Vollständiger Name: neural precursor cell expressed, developmentally down-regulated 4
• Berechnetes Molekulargewicht: 1319 aa, 149 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC152452
• Gene symbol: NEDD4
• Gene ID (NCBI): 4734
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).