NF-H/NF200 Polyklonaler Antikörper

NF-H/NF200 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(85)

Rezensionen (3)

Kat-Nr. : 18934-1-AP

Synonyme

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, 200 kDa neurofilament protein

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  WB analysis using 18934-1-AP

  Various tissues were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain tissue using 18934-1-AP

  WB result of 18934-1-AP.

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  WB analysis using 18934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat cerebellum using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 GFAP antibody (CL594-60190, Clone: 4B2E10, red), NeuN antibody (66836-1-Ig, Clone: 3A4C1, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Ratten-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 30 publications below
• IHC: See 18 publications below
• IF: See 46 publications below

Produktinformation

18934-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA NF-H/NF200 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF-H/NF200 fusion protein Ag13517
• Vollständiger Name: neurofilament, heavy polypeptide
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 200 kDa, 140-160 kDa
• GenBank-Zugangsnummer: BC014185
• Gene symbol: NF-H
• Gene ID (NCBI): 4744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.

Publikationen

Species	Application	Title
mouse	IHC	Cell Metab; Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes; Authors - Junpeng Yang
mouse	IF	Adv Sci (Weinh); GPR37 Activation Alleviates Bone Cancer Pain via the Inhibition of Osteoclastogenesis and Neuronal Hyperexcitability; Authors - Kaiyuan Wang
human	IHC	Small Methods; Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration; Authors - Shunyi Lu
rat	IHC	Biofabrication; Pulsed electromagnetic field-assisted reduced graphene oxide composite 3D printed nerve scaffold promotes sciatic nerve regeneration in rats; Authors - Zichao Wang
rat	IF	Cell Rep; TMC7 functions as a suppressor of Piezo2 in primary sensory neurons blunting peripheral mechanotransduction; Authors - Xiaoxue Zhang
		Acta Neuropathol Commun; A robust TDP-43 knock-in mouse model of ALS.; Authors - Shih-Ling Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

aidan (Verified Customer) (07-27-2024)

icc on retinal neurons (tried both mouse and human - human in pic). worked very well. would highly recommend

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: retinal neuron (mouse and human)

NF-H/NF200 Antibody Immunofluorescence validation (1:500 dilution) in retinal neuron (mouse and human) (Cat no:18934-1-AP)

FH

Paula (Verified Customer) (04-17-2024)

Works well for immunocytochemistry. Cells after neurogenic differentiation - image attached.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500

NF-H/NF200 Antibody Immunofluorescence validation (1:500 dilution) in (Cat no:18934-1-AP)

FH

Azita (Verified Customer) (06-02-2021)

Western blotting analysis of NSC34 cells using NF-H Polyclonal Antibody showed strong bonds when the antibody was used at the dilution of 1:400.

• Applications: Western Blot
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: NSC34 cells