NF-H/NF200 Polyklonaler Antikörper

NF-H/NF200 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

38

Rezensionen (1)

Kat-Nr. : 18934-1-AP

Synonyme

200 kDa neurofilament protein, NEFH, NEH, Neurofilament H, NF, NF H, NF200, NF-H

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Galerie der Validierungsdaten

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  WB analysis using 18934-1-AP

  Various tissues were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain tissue using 18934-1-AP

  WB result of 18934-1-AP.

• 

  WB analysis using 18934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18934-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 18934-1-AP (NF-H antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 18934-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 18934-1-AP (NF-H antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IHC: See 11 publications below
• IF: See 16 publications below

Produktinformation

18934-1-AP bindet in WB, IHC, IF, ELISA NF-H/NF200 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF-H/NF200 fusion protein Ag13517
• Vollständiger Name: neurofilament, heavy polypeptide
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 200 kDa, 140-160 kDa
• GenBank-Zugangsnummer: BC014185
• Gene symbol: NEFH
• Gene ID (NCBI): 4744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.

Publikationen

Species	Application	Title
mouse	IHC	Cell Metab; Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes; Authors - Junpeng Yang
human	IHC	Small Methods; Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration; Authors - Shunyi Lu
		Acta Neuropathol Commun; A robust TDP-43 knock-in mouse model of ALS.; Authors - Shih-Ling Huang
mouse	WB	PLoS Genet; Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules.; Authors - Rachid El Fatimy
mouse	IHC	Glia; ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.; Authors - Lukasz Mateusz Szewczyk
rat	IF	Int J Pharm; Berberine-loaded MSC-derived sEVs encapsulated in injectable GelMA hydrogel for spinal cord injury repair; Authors - Heng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-02-2021)

Western blotting analysis of NSC34 cells using NF-H Polyclonal Antibody showed strong bonds when the antibody was used at the dilution of 1:400.

• Applications: Western Blot
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: NSC34 cells

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