NF-M Polyklonaler Antikörper

NF-M Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 25805-1-AP

Synonyme

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

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Galerie der Validierungsdaten

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  WB analysis using 25805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25805-1-AP (NF-M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25805-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25805-1-AP (NEFM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25805-1-AP (NEFM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse brain using 25805-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25805-1-AP (NF-M antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of PC-12 using 25805-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (25805-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Hirngewebe, humanes Kolongewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe
• Erfolgreiche Detektion in FC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

25805-1-AP bindet in WB, IHC, IF, FC, ELISA NF-M und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF-M fusion protein Ag22709
• Vollständiger Name: neurofilament, medium polypeptide
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC002421
• Gene symbol: NEFM
• Gene ID (NCBI): 4741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognizes endogenous NF-M protein.

Publikationen

Species	Application	Title
canine	IF	Theranostics; Implantation of regenerative complexes in traumatic brain injury canine models enhances the reconstruction of neural networks and motor function recovery.; Authors - Jipeng Jiang
mouse, canine	IF	Biomater Sci; 3D printing collagen/heparin sulfate scaffolds boost neural network reconstruction and motor function recovery after traumatic brain injury in canine.; Authors - Jipeng Jiang
mouse	WB	Mol Autism; RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.; Authors - Natasha L Pacheco
canine	IHC	Front Cardiovasc Med; Vagus Nerve Stimulation in Early Stage of Acute Myocardial Infarction Prevent Ventricular Arrhythmias and Cardiac Remodeling.; Authors - Shuang Zhao
rat	WB	Neurosci Lett; Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.; Authors - Anwei Zhang
rat	WB	Cell Biol Int; Study of the protective effect on damaged intestinal epithelial cells of rat multilineage-differentiating stress-enduring (Muse) cells.; Authors - Dong Sun

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NF-L Polyclonal antibody

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NF-L Monoclonal antibody

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