NF-M Monoklonaler Antikörper

NF-M Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E3B12

Kat-Nr. : 66396-1-Ig

Synonyme

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 66396-1-Ig

  Lysates of rat brain, mouse brain tissues and PC-12 cells were subjected to SDS PAGE followed by western blot with 66396-1-Ig (NEFM Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NEFM antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NEFM antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of PC-12 using 66396-1-Ig

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (66396-1-Ig, Clone:2E3B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Maushirngewebe, PC-12-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:200-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66396-1-Ig bindet in WB, IHC, FC, ELISA NF-M und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NF-M fusion protein Ag22709
• Vollständiger Name: neurofilament, medium polypeptide
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC002421
• Gene symbol: NEFM
• Gene ID (NCBI): 4741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.

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