NF-M-Specific Polyklonaler Antikörper

NF-M-Specific Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20664-1-AP

Synonyme

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M-Specific

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20664-1-AP

  mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain tissue using 20664-1-AP

  WB result of 20664-1-AP.

• 

  WB analysis of mouse brain using 20664-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20664-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20664-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20664-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 20664-1-AP

  IP Result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of human brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of rat brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.

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  IHC staining of rat brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.

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  IF Staining of SH-SY5Y using 20664-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of PC-12 using 20664-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

20664-1-AP bindet in WB, IP, IHC, IF, FC, ELISA NF-M-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: neurofilament, medium polypeptide
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 140-160 kDa
• GenBank-Zugangsnummer: NM_005382
• Gene symbol: NEFM
• Gene ID (NCBI): 4741
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. The antibody is specific to NEFM.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; Neurofilament Light Regulates Axon Caliber, Synaptic Activity, and Organelle Trafficking in Cultured Human Motor Neurons.; Authors - Markus T Sainio
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu

Verwandte Produkte

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21471-1-AP

NF-H/NF200 Monoclonal antibody

60331-1-Ig

NF-L Polyclonal antibody

12998-1-AP

NF-L Monoclonal antibody

60189-1-Ig

NF-M Polyclonal antibody

25805-1-AP

NF-M Monoclonal antibody

66396-1-Ig