NEIL1 Rekombinanter Antikörper

NEIL1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240322G10

Kat-Nr. : 83376-7-RR

Synonyme

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

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  WB analysis using 83376-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83376-7-RR (NEIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 83376-7-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NEIL1 Recombinant antibody (83376-7-RR, Clone:240322G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83376-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83376-7-RR against Human NEIL1 were performed. The affinity constant is 0.521 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, COLO 320-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83376-7-RR bindet in WB, ELISA NEIL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NEIL1 fusion protein Ag2793
• Vollständiger Name: nei endonuclease VIII-like 1 (E. coli)
• Berechnetes Molekulargewicht: 390 aa, 44 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC010876
• Gene symbol: NEIL1
• Gene ID (NCBI): 79661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)