NEK2 Polyklonaler Antikörper

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• KD/KO Validated

NEK2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 24171-1-AP

Synonyme

NimA like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21

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• 

  WB analysis using 24171-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24171-1-AP (NEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 24171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24171-1-AP (NEK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24171-1-AP (NEK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24171-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEK2 antibody (24171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 7 publications below

Produktinformation

24171-1-AP bindet in WB, IHC, ELISA NEK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEK2 fusion protein Ag21476
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 2
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC043502
• Gene symbol: NEK2
• Gene ID (NCBI): 4751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Nat Commun; NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.; Authors - Xiaozhen Zhang ; KD Validated
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
mouse,human	IHC,WB	Biochim Biophys Acta Mol Cell Res; NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis; Authors - Youhua Peng ; KD Validated
human,mouse	IHC	Int J Biol Sci; NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.; Authors - Rui Bai ; KD Validated
human	WB,IHC	Mol Carcinog; NEK2 Promotes ESCC Malignant Progression by Inhibiting Cellular Senescence via the FOXM1/c-Myc/p27 Signaling Pathway; Authors - Jiachen Li ; KD Validated
human	WB,IHC	Discov Oncol; NEK2 is a potential pan-cancer biomarker and immunotherapy target; Authors - Lanyue Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works very well WB dilution 1:1000 and for immunofluorescence of paraffin embedded tissue

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: Human pulmonary artery smooth muscle cells