NEK2 Monoklonaler Antikörper

NEK2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3D7B4

Kat-Nr. : 66632-1-Ig

Synonyme

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

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  WB analysis of human peripheral blood leukocyte using 66632-1-Ig

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 66632-1-Ig

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66632-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66632-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 66632-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66632-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66632-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66632-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66632-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 66632-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human peripheral blood leukocyte, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, Ratten-Thymusgewebe, RAW 264.7-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66632-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA NEK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NEK2 fusion protein Ag21476
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 2
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC043502
• Gene symbol: NEK2
• Gene ID (NCBI): 4751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Toxicol Lett; Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.; Authors - Momoka Ota
human	WB,IF,CoIP	Cancer Cell Int; NEK2 contributes to radioresistance in esophageal squamous cell carcinoma by inducing protective autophagy via regulating TRIM21; Authors - Dong Guo ; KD Validated