NEK2 Monoklonaler Antikörper

NEK2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D7B4

Kat-Nr. : 66632-1-PBS

Synonyme

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

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  WB analysis of human peripheral blood leukocyte using 66632-1-Ig (same clone as 66632-1-PBS)

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat thymus using 66632-1-Ig (same clone as 66632-1-PBS)

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66632-1-Ig (same clone as 66632-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66632-1-Ig (same clone as 66632-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66632-1-Ig (same clone as 66632-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66632-1-Ig (same clone as 66632-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66632-1-Ig (same clone as 66632-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66632-1-Ig (same clone as 66632-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66632-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NEK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NEK2 fusion protein Ag21476
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 2
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC043502
• Gene symbol: NEK2
• Gene ID (NCBI): 4751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.