NEK4 Polyklonaler Antikörper

NEK4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31389-1-PBS

Synonyme

STK2, pp12301, NRK2, NimA related protein kinase 4

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  WB analysis using 31389-1-AP (same clone as 31389-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31389-1-AP (NEK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31389-1-AP (NEK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31389-1-AP (NEK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31389-1-PBS bindet in WB, IHC, Indirect ELISA NEK4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEK4 fusion protein Ag34929
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 4
• Berechnetes Molekulargewicht: 781 aa, 88 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC063044
• Gene symbol: NEK4
• Gene ID (NCBI): 6787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.