NEK4 Rekombinanter Antikörper

NEK4 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242081B9

Kat-Nr. : 84654-4-PBS

Synonyme

242081B9, NimA related protein kinase 4, NRK2, pp12301, STK2

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  WB analysis using 84654-4-RR (same clone as 84654-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84654-4-RR (NEK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using NEK4 antibody (84654-4-RR, Clone: 242081B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84654-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84654-4-RR against Human NEK4 were performed. The affinity constant is 92.8 pM.

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Geprüfte Anwendungen

Produktinformation

84654-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA NEK4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NEK4 fusion protein Ag34929
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 4
• Berechnetes Molekulargewicht: 781 aa, 88 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC063044
• Gene symbol: NEK4
• Gene ID (NCBI): 6787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.