NEK7 Polyklonaler Antikörper

NEK7 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31422-1-PBS

Synonyme

NimA related protein kinase 7

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  WB analysis using 31422-1-AP (same clone as 31422-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31422-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 31422-1-AP (same clone as 31422-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31422-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 31422-1-AP (same clone as 31422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31422-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 31422-1-AP (same clone as 31422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31422-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 31422-1-AP (same clone as 31422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31422-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 31422-1-AP (same clone as 31422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31422-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 31422-1-AP (same clone as 31422-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEK7 antibody (31422-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31422-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31422-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NEK7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEK7 fusion protein Ag33629
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 7
• Berechnetes Molekulargewicht: 35
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: NM_133494
• Gene symbol: NEK7
• Gene ID (NCBI): 140609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian NIMA-related kinases (NEKs) represent a family of serine/threonine kinases, named NEK1-NEK11, which are implicated in the control of several aspects of mitosis and are involved in non-mitotic functions (PMID: 33364979). NIMA-related kinase 7 (NEK7) is the smallest NIMA-related kinase (NEK) in mammals. NEK7 is identified as a highly conserved serine or threonine kinase, which is not only crucial for mitosis entry, cell cycle progression, cell division, and mitotic process, but also expressed in brain, heart, lung, liver, spleen, and other issues (PMID: 31787755).