NELF-A Polyklonaler Antikörper

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• KD/KO Validated

NELF-A Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10456-1-PBS

Synonyme

WHSC2, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, NELF A

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  WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

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  WB analysis using 10456-1-AP (same clone as 10456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 10456-1-AP (same clone as 10456-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10456-1-AP (same clone as 10456-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)

  IP result of anti-NELF-A (IP:10456-1-AP, 4ug; Detection:10456-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELF-A antibody (10456-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10456-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA NELF-A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NELF-A fusion protein Ag0708
• Vollständiger Name: Wolf-Hirschhorn syndrome candidate 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC002764
• Gene symbol: NELFA
• Gene ID (NCBI): 7469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WHSC2, also known as NELF-A, is a component of the Negative ELongation Factor complex, which inhibits RNA polymerase II progression early during elongation (a process referred to as 'promoter proximal pausing'), thereby altering expression of its target genes in a positive and negative manner [PMID:19245807]. This complex acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 and SPT5, to cause transcriptional pausing of RNA polymerase II[PMID:12612062].

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