NELF Polyklonaler Antikörper

NELF Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12244-1-AP

Synonyme

NSMF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor

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  WB analysis of HeLa using 12244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12244-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12244-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12244-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12244-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12244-1-AP (NELF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12244-1-AP (NELF antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 12244-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12244-1-AP (NELF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Kolongewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12244-1-AP bindet in WB, IHC, IF/ICC, ELISA NELF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NELF fusion protein Ag2888
• Vollständiger Name: nasal embryonic LHRH factor
• Berechnetes Molekulargewicht: 507 aa, 56 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC016862
• Gene symbol: NELF
• Gene ID (NCBI): 26012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSMF, also named as NELF (Nasal embryonic LHRH factor), couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. NSMF has some isoforms with MW 44 kDa and 55-60 kDa. This antibody recognizes all the isoforms.