NELF Rekombinanter Antikörper

NELF Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240482G1

Kat-Nr. : 83490-2-PBS

Synonyme

NSMF, 240482G1, Nasal embryonic LHRH factor, Nasal embryonic luteinizing hormone-releasing hormone factor, NMDA receptor synaptonuclear signaling and neuronal migration factor

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  WB analysis using 83490-2-RR (same clone as 83490-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83490-2-RR (NELF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83490-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00490-1

  Sandwich ELISA standard curve of MP00490-1, Human NELF Recombinant Matched Antibody Pair - PBS only. 83490-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34902. 83490-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

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  Sample test of MP00490-1

  The mean NELF concentration was determined to be 45.6 ng/mL in HepG2 cell extract based on a 2.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP00490-1

  Cytometric bead array standard curve of MP00490-1, NELF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83490-1-PBS. Detection antibody: 83490-2-PBS. Standard: Ag34902. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83490-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83490-2-RR against Human NELF were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83490-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test NELF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NELF fusion protein Ag34902
• Vollständiger Name: nasal embryonic LHRH factor
• Berechnetes Molekulargewicht: 507 aa, 56 kDa
• GenBank-Zugangsnummer: BC016862
• Gene symbol: NELF
• Gene ID (NCBI): 26012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.