NESG1 Polyklonaler Antikörper

NESG1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21077-1-AP

Synonyme

CCDC19, CFAP45

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  WB analysis of rat testis using 21077-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21077-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

21077-1-AP bindet in WB, IHC, ELISA NESG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NESG1 fusion protein Ag15257
• Vollständiger Name: coiled-coil domain containing 19
• Berechnetes Molekulargewicht: 551 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC027874
• Gene symbol: CCDC19
• Gene ID (NCBI): 25790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC19, also named as NESG1, is specifically expressed in human nasopharynx and trachea, and decreased or absent in nasopharyngeal carcinoma (NPC) tissues and cell lines. In addition, the levels of NESG1 protein are significantly greater in the low-grade NPC tissues than that in the high-grade NPC tissues. NESG1 also significantly regulats the expression of cell cycle regulators CCNA1 and p21. NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Loss of connective tissue growth factor as an unfavorable prognosis factor activates miR-18b by PI3K/AKT/C-Jun and C-Myc and promotes cell growth in nasopharyngeal carcinoma.; Authors - X Yu
human	WB,IHC	J Cell Mol Med; Candidate tumour suppressor CCDC19 regulates miR-184 direct targeting of C-Myc thereby suppressing cell growth in non-small cell lung cancers.; Authors - Zhen Liu ; KD Validated