NEU2 Rekombinanter Antikörper

NEU2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240685B3

Kat-Nr. : 83934-1-PBS

Synonyme

240685B3, Cytosolic sialidase, EC:3.2.1.18, N acetyl alpha neuraminidase 2, N-acetyl-alpha-neuraminidase 2

---

• 

  WB analysis of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 83934-1-RR (NEU2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83934-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83934-1-RR against Human NEU2 were performed. The affinity constant is 2.76 nM.

---

Geprüfte Anwendungen

Produktinformation

83934-1-PBS bindet in WB, IHC, ELISA NEU2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NEU2 fusion protein Ag17386
• Vollständiger Name: sialidase 2 (cytosolic sialidase)
• Berechnetes Molekulargewicht: 380 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC107053
• Gene symbol: NEU2
• Gene ID (NCBI): 4759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)