NEU3 Monoklonaler Antikörper

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• KD/KO Validated

NEU3 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C9C5

Kat-Nr. : 67098-1-PBS

Synonyme

cDNA FLJ78026, FLJ12388, NEU3, NEU3 protein, SIAL3

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  WB analysis of PC-3 using 67098-1-Ig (same clone as 67098-1-PBS)

  WB result of NEU3 antibody (67098-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEU3 transfected PC-3 cells. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67098-1-Ig (same clone as 67098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67098-1-Ig (same clone as 67098-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 67098-1-Ig (same clone as 67098-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67098-1-Ig (same clone as 67098-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67098-1-Ig (same clone as 67098-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67098-1-Ig (same clone as 67098-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 67098-1-Ig (same clone as 67098-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67098-1-Ig (NEU3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67098-1-Ig (same clone as 67098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67098-1-Ig (same clone as 67098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67098-1-Ig (same clone as 67098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67098-1-Ig (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67098-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67098-1-PBS bindet in WB, IHC, Indirect ELISA NEU3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NEU3 fusion protein Ag27272
• Vollständiger Name: sialidase 3 (membrane sialidase)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa
• GenBank-Zugangsnummer: NM_006656.5
• Gene symbol: NEU3
• Gene ID (NCBI): 10825
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.