NEURL Polyklonaler Antikörper

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• KD/KO Validated

NEURL Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 18898-1-AP

Synonyme

h neu, h neuralized 1, neu, neu 1, Neuralized like protein 1A, NEURL, NEURL1, NEURL1A, RING finger protein 67, RNF67

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 18898-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18898-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 18898-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18898-1-AP

  IP Result of anti-NEURL (IP:18898-1-AP, 4ug; Detection:18898-1-AP 1:600) with mouse brain tissue lysate 2560ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, MCF-7-Zellen, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

18898-1-AP bindet in WB, IP, ELISA NEURL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEURL fusion protein Ag13531
• Vollständiger Name: neuralized homolog (Drosophila)
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60-62 kDa
• GenBank-Zugangsnummer: BC026336
• Gene symbol: NEURL
• Gene ID (NCBI): 9148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEURL(Neuralized-like protein 1A) is also named as NEURL1, NEURL1A, RNF67.Neurl1 is a RING-dependent E3 ubiquitin ligase that can regulate Notch signaling in mammals and it expression promotes lysosomal degradation of Jagged1 in cells and blocks signaling from Jagged1-expressing cells to neighboring Notch-expressing cells. Neurl-dependent turnover of Jagged1 and inhibition of Notch signaling depends upon N-terminal myristoylation and plasma membrane localization of Neurl1(PMID:18077452).This antibody is specific to NEURL.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice.; Authors - Kun Zhang ; KD Validated