NEURL Polyklonaler Antikörper

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NEURL Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18898-1-PBS

Synonyme

neu, h-neuralized 1, h neuralized 1, h neu, EC:2.3.2.27

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  WB analysis of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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  WB analysis using 18898-1-AP (same clone as 18898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 18898-1-AP (same clone as 18898-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 18898-1-AP (same clone as 18898-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  IP result of anti-NEURL (IP:18898-1-AP, 4ug; Detection:18898-1-AP 1:600) with mouse brain tissue lysate 2560ug. This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18898-1-AP (NEURL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18898-1-AP (same clone as 18898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18898-1-AP (NEURL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18898-1-AP (same clone as 18898-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NEURL antibody (18898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18898-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18898-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NEURL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEURL fusion protein Ag13531
• Vollständiger Name: neuralized homolog (Drosophila)
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60-62 kDa
• GenBank-Zugangsnummer: BC026336
• Gene symbol: NEURL
• Gene ID (NCBI): 9148
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEURL(Neuralized-like protein 1A) is also named as NEURL1, NEURL1A, RNF67.Neurl1 is a RING-dependent E3 ubiquitin ligase that can regulate Notch signaling in mammals and it expression promotes lysosomal degradation of Jagged1 in cells and blocks signaling from Jagged1-expressing cells to neighboring Notch-expressing cells. Neurl-dependent turnover of Jagged1 and inhibition of Notch signaling depends upon N-terminal myristoylation and plasma membrane localization of Neurl1(PMID:18077452).This antibody is specific to NEURL.