NEUROD1 Monoklonaler Antikörper

NEUROD1 Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3E10F1

Kat-Nr. : 66691-1-PBS

Synonyme

3E10F1, bHLHa3, Class A basic helix-loop-helix protein 3, NEUROD

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  WB analysis of Y79 using 66691-1-Ig (same clone as 66691-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66691-1-Ig (NEUROD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66691-1-Ig (same clone as 66691-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66691-1-Ig (NEUROD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66691-1-Ig (same clone as 66691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66691-1-Ig (NEUROD1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66691-1-Ig (same clone as 66691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66691-1-Ig (NEUROD1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 66691-1-Ig (same clone as 66691-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.5 ug Anti-Human NEUROD1 (66691-1-Ig, Clone:3E10F1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66691-1-PBS bindet in WB, IHC, FC (Intra), ELISA NEUROD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NEUROD1 fusion protein Ag27606
• Vollständiger Name: neurogenic differentiation 1
• Berechnetes Molekulargewicht: 356 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC009046
• Gene symbol: NEUROD1
• Gene ID (NCBI): 4760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.