NF-κB p65 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

NF-κB p65 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

4C7

Kat-Nr. : 80979-1-PBS

Synonyme

NF κB, p65, p65; RELA, RELA, 4C7

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  WB analysis using 80979-1-RR (same clone as 80979-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80979-1-RR (NF-κB p65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80979-1-RR (same clone as 80979-1-PBS)

  WB result of NF-κB p65 antibody (80979-1-RR; 1:13000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NF-κB p65 transfected HEK-293 cells. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 80979-1-RR (same clone as 80979-1-PBS)

  WB result of NF-κB p65 antibody (80979-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NF-κB p65 transfected HEK-293 cells. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 80979-1-RR (same clone as 80979-1-PBS)

  IP result of anti-NF-κB p65 (IP:80979-1-RR, 4ug; Detection:80979-1-RR 1:4000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NF-κB p65 antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NF-κB p65 antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-1080 using 80979-1-RR (same clone as 80979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using NF-κB p65 antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NF-κB p65 (80979-1-RR, Clone:4C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 80979-1-RR (same clone as 80979-1-PBS)

  Chromatin was prepared from HeLa cells either non-treated or treated with hTNF-α (30 ng/ml, 1 h). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of NF-κB p65 (80979-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80979-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80979-1-RR against Human NF-κB p65 were performed. The affinity constant is 3.57 nM.

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Geprüfte Anwendungen

Produktinformation

80979-1-PBS bindet in WB, IF/ICC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR NF-κB p65 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NF-κB p65 fusion protein Ag1199
• Vollständiger Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC011603
• Gene symbol: NF-κB p65
• Gene ID (NCBI): 5970
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit monoclonal antibody raised against residues near the N terminus of human RELA.