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NF-κB p65 Polyklonaler Antikörper

NF-κB p65 Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10745

Synonyme

NFKB3, p65, RELA, Transcription factor p65



Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10745 bindet in IF NF-κB p65 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NF-κB p65 fusion protein Ag1199
Vollständiger Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Berechnetes Molekulargewicht 65 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC011603
Gene symbol RELA
Gene ID (NCBI) 5970
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 NF-κB p65 antibody CL488-10745Protokoll herunterladen
Standard-Protokolle
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