NF-κB p65 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NF-κB p65 Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

Kat-Nr. : CL594-10745

Synonyme

NF κB, p65, p65; RELA, RELA, NF Kappa B

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  IF Staining of HepG2 using CL594-10745

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:100, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green). DAPI (Blue).

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  IF Staining of HT-1080 using CL594-10745

  Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-10745

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, TNF alpha treated HT-1080 cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL594-10745 bindet in IF/ICC NF-κB p65 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF-κB p65 fusion protein Ag1199
• Vollständiger Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC011603
• Gene symbol: NF-κB p65
• Gene ID (NCBI): 5970
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Publikationen

Species	Application	Title
mouse	IF	Front Med (Lausanne); New, potent, small molecule agonists of tyrosine kinase receptors attenuate dry eye disease; Authors - Zhiyuan Yu
mouse	IF	Invest Ophthalmol Vis Sci; Induction of Innate Inflammatory Pathways in the Corneal Epithelium in the Desiccating Stress Dry Eye Model; Authors - Zhiyuan Yu