NF-H/NF200 Polyklonaler Antikörper

NF-H/NF200 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18934-1-PBS

Synonyme

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, 200 kDa neurofilament protein

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  WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

  Various tissues were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain tissue using 18934-1-AP (same clone as 18934-1-PBS)

  WB result of 18934-1-AP. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IF Staining of rat cerebellum using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 GFAP antibody (CL594-60190, Clone: 4B2E10, red), NeuN antibody (66836-1-Ig, Clone: 3A4C1, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18934-1-PBS bindet in WB, IHC, IF-P, IF-Fro, Indirect ELISA NF-H/NF200 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF-H/NF200 fusion protein Ag13517
• Vollständiger Name: neurofilament, heavy polypeptide
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 200 kDa, 140-160 kDa
• GenBank-Zugangsnummer: BC014185
• Gene symbol: NF-H
• Gene ID (NCBI): 4744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.