Neurofibromin 2 Polyklonaler Antikörper

Neurofibromin 2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10659-1-AP

Synonyme

NF2, Neurofibromin-2, Moesin-ezrin-radixin-like protein, Merlin

---

• 

  WB analysis using 10659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10659-1-AP (Neurofibromin 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10659-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (Neurofibromin 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10659-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (Neurofibromin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10659-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (Neurofibromin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10659-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (Neurofibromin 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 10659-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10659-1-AP (Neurofibromin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Nierengewebe, Mausnierengewebe, NIH/3T3-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10659-1-AP bindet in WB, IHC, ELISA Neurofibromin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neurofibromin 2 fusion protein Ag1013
• Vollständiger Name: neurofibromin 2 (merlin)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 64-66 kDa
• GenBank-Zugangsnummer: BC007279
• Gene symbol: NF2
• Gene ID (NCBI): 4771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling; Authors - Wenbo Ma