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NF2 Polyklonaler Antikörper

NF2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21686-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis using 21686-1-AP (same clone as 21686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of HepG2 using 21686-1-AP (same clone as 21686-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of MCF-7 using 21686-1-AP (same clone as 21686-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of HEK-293 using 21686-1-AP (same clone as 21686-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of Jurkat using 21686-1-AP (same clone as 21686-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of SH-SY5Y using 21686-1-AP (same clone as 21686-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of NIH/3T3 using 21686-1-AP (same clone as 21686-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IP experiment of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    IP result of anti-NF2 (IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human meningioma tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human meningioma tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NF2 antibody (21686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21686-1-AP (NF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21686-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA NF2 und zeigt Reaktivität mit human, Hund, Maus, Ratten

Getestete Reaktivität human, Hund, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NF2 fusion protein Ag16265
Vollständiger Name neurofibromin 2 (merlin)
Berechnetes Molekulargewicht 595 aa, 70 kDa
Beobachtetes Molekulargewicht 66-70 kDa
GenBank-ZugangsnummerBC020257
Gene symbol NF2
Gene ID (NCBI) 4771
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.