NF-H/NF200 Monoklonaler Antikörper

NF-H/NF200 Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(26)

CloneNo.

1A3C7

Kat-Nr. : 60331-1-Ig

Synonyme

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, 1A3C7



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHausschwein-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHCRatten-Cerebellum-Gewebe, Rattenhirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PRattenhirngewebe
Erfolgreiche Detektion in IF-FroRattenhirngewebe
Erfolgreiche Detektion in FC (Intra)SH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:4000-1:16000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Immunfluoreszenz (IF)-FROIF-FRO : 1:400-1:1600
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60331-1-Ig bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA NF-H/NF200 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen NF-H/NF200 fusion protein Ag13517
Vollständiger Name neurofilament, heavy polypeptide
Berechnetes Molekulargewicht 112 kDa
Beobachtetes Molekulargewicht 200 kDa
GenBank-ZugangsnummerBC014185
Gene symbol NF-H
Gene ID (NCBI) 4744
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for NF-H/NF200 antibody 60331-1-IgProtokoll herunterladen
IHC protocol for NF-H/NF200 antibody 60331-1-IgProtokoll herunterladenl
IF protocol for NF-H/NF200 antibody 60331-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIF

Bioact Mater

Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration

Authors - Yiting Sun
mouseIF

ACS Nano

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia

Authors - Shishi Shen
mouse,humanIF

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li
ratIF

Biomaterials

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.

Authors - Xianzhen Dong
ratIF

J Neuroinflammation

Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.

Authors - Qing Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Reyes (Verified Customer) (07-02-2024)

This neurofilament antibody worked perfectly showing my neurons in human brain FFPE cortex

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human Brain Cortex
NF-H/NF200 Antibody Immunofluorescence validation (1:200 dilution) in Human Brain Cortex (Cat no:60331-1-Ig)