NF-H/NF200 Monoklonaler Antikörper
NF-H/NF200 Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
1A3C7
Kat-Nr. : 60331-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Hausschwein-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe |
Erfolgreiche Detektion in IHC | Ratten-Cerebellum-Gewebe, Rattenhirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Rattenhirngewebe |
Erfolgreiche Detektion in IF-Fro | Rattenhirngewebe |
Erfolgreiche Detektion in FC (Intra) | SH-SY5Y-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:4000-1:16000 |
Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 |
Immunfluoreszenz (IF)-FRO | IF-FRO : 1:400-1:1600 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 3 publications below |
IHC | See 3 publications below |
IF | See 21 publications below |
Produktinformation
60331-1-Ig bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA NF-H/NF200 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | NF-H/NF200 fusion protein Ag13517 |
Vollständiger Name | neurofilament, heavy polypeptide |
Berechnetes Molekulargewicht | 112 kDa |
Beobachtetes Molekulargewicht | 200 kDa |
GenBank-Zugangsnummer | BC014185 |
Gene symbol | NF-H |
Gene ID (NCBI) | 4744 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for NF-H/NF200 antibody 60331-1-Ig | Protokoll herunterladen |
IHC protocol for NF-H/NF200 antibody 60331-1-Ig | Protokoll herunterladenl |
IF protocol for NF-H/NF200 antibody 60331-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Bioact Mater Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration | ||
ACS Nano Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Biomaterials Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization. | ||
J Neuroinflammation Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (07-02-2024) | This neurofilament antibody worked perfectly showing my neurons in human brain FFPE cortex
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