NF45 Polyklonaler Antikörper

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• KD/KO Validated

NF45 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 14714-1-AP

Synonyme

ILF2, Interleukin enhancer-binding factor 2, Nuclear factor of activated T-cells 45 kDa, PRO3063

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  WB analysis using 14714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14714-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14714-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14714-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14714-1-AP (NF45 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

14714-1-AP bindet in WB, IHC, IF, IP, ELISA NF45 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF45 fusion protein Ag6436
• Vollständiger Name: interleukin enhancer binding factor 2, 45kDa
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC000382
• Gene symbol: ILF2
• Gene ID (NCBI): 3608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

Publikationen

Species	Application	Title
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
mouse,human	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
		Analyst; An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.; Authors - Yu Yanyan Y
human	WB,IF	Virol J; Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication.; Authors - Xuexia Wen
human	WB	J Exp Clin Cancer Res; LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex; Authors - Dandan Li
human	WB	Nat Cell Biol; Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing; Authors - Guokai Yan