NF45 Polyklonaler Antikörper

NF45 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24572-1-AP

Synonyme

ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2

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  WB analysis of K-562 using 24572-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24572-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24572-1-AP (NF45 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:200.

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.

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  IF Staining of HepG2 using 24572-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24572-1-AP (NF45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24572-1-AP bindet in WB, IHC, IF/ICC, ELISA NF45 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF45 fusion protein Ag21480
• Vollständiger Name: interleukin enhancer binding factor 2, 45kDa
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC000382
• Gene symbol: ILF2
• Gene ID (NCBI): 3608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].