NF90/ILF3 Monoklonaler Antikörper

NF90/ILF3 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H10G3

Kat-Nr. : 68213-1-PBS

Synonyme

ILF3, IL-F3, DRBP76, DRBF, CBTF

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  WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 68213-1-Ig (same clone as 68213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50472-3

  Cytometric bead array standard curve of MP50472-3, NF90,ILF3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68213-1-PBS. Detection antibody: 68213-4-PBS. Standard:Ag30868. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68213-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA NF90/ILF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NF90/ILF3 fusion protein Ag30868
• Vollständiger Name: interleukin enhancer binding factor 3, 90kDa
• Berechnetes Molekulargewicht: 894 aa, 95 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 110 kDa
• GenBank-Zugangsnummer: BC064836
• Gene symbol: ILF3
• Gene ID (NCBI): 3609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.