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NFATC3 Polyklonaler Antikörper
NFATC3 Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IHC, IF, IP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18222-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Jurkat-Zellen, K-562-Zellen | 
| Erfolgreiche IP | K-562-Zellen | 
| Erfolgreiche Detektion in IHC | Maushodengewebe,  humanes Hodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 2 publications below | 
| WB | See 23 publications below | 
| IHC | See 2 publications below | 
| IF | See 7 publications below | 
| IP | See 1 publications below | 
| ChIP | See 4 publications below | 
Produktinformation
18222-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA NFATC3 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | NFATC3 fusion protein Ag12985 | 
| Vollständiger Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 | 
| Berechnetes Molekulargewicht | 116 kDa | 
| Beobachtetes Molekulargewicht | 130-170 kDa | 
| GenBank-Zugangsnummer | BC001050 | 
| Gene symbol | NFATC3 | 
| Gene ID (NCBI) | 4775 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for NFATC3 antibody 18222-1-AP | Protokoll herunterladen | 
| IHC protocol for NFATC3 antibody 18222-1-AP | Protokoll herunterladenl | 
| IP protocol for NFATC3 antibody 18222-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Immunity Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation. | ||
| Gastroenterology Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1. | ||
| Cell Death Differ Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3. | ||
| Oncogene Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells. | ||
| Cancer Lett TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway. | ||
| Cell Biol Toxicol HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells | 





