NFATC3 Polyklonaler Antikörper

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• KD/KO Validated

NFATC3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(32)

Kat-Nr. : 18222-1-AP

Synonyme

NF AT4, NF ATc3, NFAT4, NF-AT4, NF-AT4c

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  WB analysis using 18222-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18222-1-AP (NFATC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18222-1-AP

  IP result of anti-NFATC3 (IP:18222-1-AP, 4ug; Detection:18222-1-AP 1:1000) with K-562 cells lysate 1760 ug.

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  IHC staining of mouse testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18222-1-AP (NFATC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18222-1-AP (NFATC3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18222-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18222-1-AP (NFATC3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below

Produktinformation

18222-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA NFATC3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFATC3 fusion protein Ag12985
• Vollständiger Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Berechnetes Molekulargewicht: 116 kDa
• Beobachtetes Molekulargewicht: 130-170 kDa
• GenBank-Zugangsnummer: BC001050
• Gene symbol: NFATC3
• Gene ID (NCBI): 4775
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)

Publikationen

Species	Application	Title
	ChIP	Immunity; Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.; Authors - Hongxu Xian
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
mouse	WB,IP	Cell Death Differ; Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3.; Authors - Meenakshi Basu-Shrivastava
human	WB	Oncogene; Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.; Authors - Mengyi Lao
human	WB	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin
human	WB	Cell Biol Toxicol; HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells; Authors - Hanting Wang