NFIA Monoklonaler Antikörper

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• KD/KO Validated

NFIA Monoklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3A6D7

Kat-Nr. : 66999-1-Ig

Synonyme

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

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Galerie der Validierungsdaten

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  WB analysis using 66999-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of MCF-7 using 66999-1-Ig

  WB result of NFIA antibody (66999-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFIA transfected MCF-7 cells.

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  WB analysis of HeLa using 66999-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66999-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 66999-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66999-1-Ig

  IP result of anti-NFIA (IP:66999-1-Ig, 5ug; Detection:66999-1-Ig 1:500) with HeLa cells lysate 1600 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, Hausschwein-Hirngewebe, Jurkat-Zellen, Kaninchenhirngewebe, Maus-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66999-1-Ig bindet in WB, IP, ELISA NFIA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFIA fusion protein Ag17873
• Vollständiger Name: nuclear factor I/A
• Berechnetes Molekulargewicht: 498 aa, 55 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC022264
• Gene symbol: NFIA
• Gene ID (NCBI): 4774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)