NFIA Monoklonaler Antikörper

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• KD/KO Validated

NFIA Monoklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A6D7

Kat-Nr. : 66999-1-PBS

Synonyme

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

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  WB analysis using 66999-1-Ig (same clone as 66999-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66999-1-Ig (same clone as 66999-1-PBS)

  WB result of NFIA antibody (66999-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFIA transfected MCF-7 cells. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 66999-1-Ig (same clone as 66999-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66999-1-Ig (same clone as 66999-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66999-1-Ig (same clone as 66999-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 66999-1-Ig (same clone as 66999-1-PBS)

  IP result of anti-NFIA (IP:66999-1-Ig, 5ug; Detection:66999-1-Ig 1:500) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66999-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66999-1-PBS bindet in WB, IP, Indirect ELISA NFIA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFIA fusion protein Ag17873
• Vollständiger Name: nuclear factor I/A
• Berechnetes Molekulargewicht: 498 aa, 55 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC022264
• Gene symbol: NFIA
• Gene ID (NCBI): 4774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)