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NFIA Monoklonaler Antikörper
NFIA Monoklonal Antikörper für
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Kaninchen, Maus, Ratte
Anwendung
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3A6D7
Kat-Nr. : CL488-66999
Synonyme
Geprüfte Anwendungen
Produktinformation
CL488-66999 bindet in NFIA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Kaninchen, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | NFIA fusion protein Ag17873 |
| Vollständiger Name | nuclear factor I/A |
| Berechnetes Molekulargewicht | 498 aa, 55 kDa |
| Beobachtetes Molekulargewicht | 60-70 kDa |
| GenBank-Zugangsnummer | BC022264 |
| Gene symbol | NFIA |
| Gene ID (NCBI) | 4774 |
| Konjugation | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)
