NFIA Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

NFIA Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A6D7

Kat-Nr. : CL488-66999

Synonyme

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

---

Galerie der Validierungsdaten

• 

  FC experiment of HeLa using CL488-66999

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NFIA (CL488-66999, Clone:3A6D7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66999 bindet in FC (Intra) NFIA und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFIA fusion protein Ag17873
• Vollständiger Name: nuclear factor I/A
• Berechnetes Molekulargewicht: 498 aa, 55 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC022264
• Gene symbol: NFIA
• Gene ID (NCBI): 4774
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)