NFIB Polyklonaler Antikörper

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NFIB Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29898-1-PBS

Synonyme

NF1-B, NF1 B, NF I/B, HMGIC/NFIB, CTF

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  WB analysis using 29898-1-AP (same clone as 29898-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29898-1-AP (NFIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 29898-1-AP (same clone as 29898-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29898-1-AP (NFIB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29898-1-AP (same clone as 29898-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 29898-1-AP (same clone as 29898-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NFIB (29898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 29898-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29898-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA NFIB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFIB fusion protein Ag31946
• Vollständiger Name: nuclear factor I/B
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 45-65 kDa
• GenBank-Zugangsnummer: BC001283
• Gene symbol: NFIB
• Gene ID (NCBI): 4781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFIB, also named as Nuclear factor 1, is a 420 amino acid protein, which belongs to the CTF/NF-I family and may bind DNA as a homodimer. NFIB recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. NFIB also exists in multiple isoforms in prostate cancer cells, with average molecular weights of 62 kDa, 57 kDa, and 49 kDa (PMID: 32692871).