NFIC Polyklonaler Antikörper

NFIC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16399-1-AP

Synonyme

NFI-C, NF-I/C, NF1-C, NF I, CTF

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  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16399-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16399-1-AP

  IP result of anti-NFIC (IP:16399-1-AP, 4ug; Detection:16399-1-AP 1:1000) with HeLa cells lysate 3600ug.

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  IHC staining of human liver cancer using 16399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 16399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using NFIC antibody (16399-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

16399-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NFIC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFIC fusion protein Ag9494
• Vollständiger Name: nuclear factor I/C (CCAAT-binding transcription factor)
• Berechnetes Molekulargewicht: 428aa,48 kDa; 508aa,57 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC012120
• Gene symbol: NFIC
• Gene ID (NCBI): 4782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Publikationen

Species	Application	Title
mouse	IHC	J Dent Res; Essential Role of IFT140 in Promoting Dentinogenesis.; Authors - G Li
human	WB,IHC	J Bone Miner Res; METTL3-mediated m6 A mRNA methylation modulates tooth root formation via affecting NFIC translation.; Authors - Rui Sheng
human,mouse	WB,IHC,IP	Int J Oral Sci; USP34 regulates tooth root morphogenesis by stabilizing NFIC.; Authors - Shuang Jiang
bovine	WB,IF	J Agric Food Chem; Knockdown of NFIC Promotes Bovine Myoblast Proliferation through the CENPF/CDK1 Axis; Authors - Jianfang Wang ; KD Validated
human	WB,IP,IHC	Cancer Cell Int; NFIC mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to represses malignant phenotype of non-small cell lung cancer cells; Authors - Kesong Shi
human	WB	Mol Carcinog; Neuroplastin-β mediates S100A8/A9-induced lung cancer disseminative progression.; Authors - I Wayan Sumardika