NFIC Monoklonaler Antikörper

NFIC Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11A5

Kat-Nr. : 68318-1-Ig

Synonyme

NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor

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  WB analysis using 68318-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68318-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 68318-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFIC Monoclonal antibody (68318-1-Ig, Clone:1A11A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, 4T1-Zellen, HeLa-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68318-1-Ig bindet in WB, FC (Intra), ELISA NFIC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFIC fusion protein Ag9472
• Vollständiger Name: nuclear factor I/C (CCAAT-binding transcription factor)
• Berechnetes Molekulargewicht: 428aa,48 kDa; 508aa,57 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC012120
• Gene symbol: NFIC
• Gene ID (NCBI): 4782
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.