NFIX Monoklonaler Antikörper

NFIX Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E8A1

Kat-Nr. : 67983-1-PBS

Synonyme

CTF, NF I/X, NF1 X, NF1A, NFI X, NFIX, Nuclear factor 1 X type, Nuclear factor 1/X, Nuclear factor I/X, TGGCA binding protein

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  WB analysis using 67983-1-Ig (same clone as 67983-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 67983-1-Ig (same clone as 67983-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67983-1-Ig (same clone as 67983-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67983-1-Ig (same clone as 67983-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67983-1-Ig (NFIX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67983-1-Ig (same clone as 67983-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67983-1-Ig (same clone as 67983-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67983-1-Ig (NFIX antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67983-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67983-1-PBS bindet in WB, IHC, Indirect ELISA NFIX und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFIX fusion protein Ag31447
• Vollständiger Name: nuclear factor I/X (CCAAT-binding transcription factor)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 45kDa, 50kDa
• GenBank-Zugangsnummer: NM_001271043
• Gene symbol: NFIX
• Gene ID (NCBI): 4784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFIX, also named CTF, belongs to the CTF/NF-I family. NFIX recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. NFIX is individually capable of activating transcription and replication.