NFKB1,p105 Polyklonaler Antikörper

NFKB1,p105 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 23576-1-AP

Synonyme

DNA binding factor KBF1, EBP 1, KBF1, NF kappa B, NFKB p105, NFKB p50, NFKB1, NFKB1,p105, NFKB1,p105,p50, nfkb1a, p105, p50

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 23576-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1,p105 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 23576-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23576-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23576-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 23576-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 23576-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 23576-1-AP (NFKB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 23576-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 23576-1-AP (NFKB1,p105 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 23576-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 23576-1-AP (NFKB1,p105 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

23576-1-AP bindet in WB, IHC, IF, chIP, ELISA NFKB1,p105 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFKB1,p105 fusion protein Ag20297
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC051765
• Gene symbol: NFKB1
• Gene ID (NCBI): 4790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind p105 isoforms of NFKB1.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Single cell whole genome sequencing reveals that NFKB1 mutation affects radiotherapy sensitivity in cervical cancer.; Authors - Dong Yang
human	chIP	In Vitro Cell Dev Biol Anim; NFKB1-miR-612-FAIM2 pathway regulates tumorigenesis in neurofibromatosis type 1.; Authors - Meng Wang