NFKB1,p105,p50 Monoklonaler Antikörper

NFKB1,p105,p50 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

2G1E3

Kat-Nr. : 66992-1-Ig

Synonyme

NFKB1, KBF1, EBP-1, EBP 1, DNA-binding factor KBF1

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  WB analysis using 66992-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66992-1-Ig (NFKB1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66992-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66992-1-Ig (NFKB1,p105,p50 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66992-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFKB1,p105,p50 antibody (66992-1-Ig, Clone: 2G1E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66992-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (66992-1-Ig, Clone:2G1E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Appendizitis-Gewebe, humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 4 publications below
• ChIP: See 1 publications below

Produktinformation

66992-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ChIP, ELISA NFKB1,p105,p50 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFKB1,p105,p50 fusion protein Ag5832
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 105 kDa
• GenBank-Zugangsnummer: BC051765
• Gene symbol: NFKB1
• Gene ID (NCBI): 4790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.

Publikationen

Species	Application	Title
human	WB	Commun Biol; EZH2 elicits CD8+ T-cell desert in esophageal squamous cell carcinoma via suppressing CXCL9 and dendritic cells; Authors - Chun-Yan Zhu
mouse	IF	J Ethnopharmacol; Sub-chronic exposure to realgar induces liver injury via upregulating the TXNIP/NLRP3 pathway and disturbing bile acid homeostasis in mice; Authors - Aihong Li
mouse	WB	Chem Biol Drug Des; Caffeic acid-grafted chitooligosaccharides downregulate MAPK and NF-kB in RAW264.7 cells; Authors - Van-Hoai Bui
human	WB	Chem Biol Interact; Cordycepin inhibits glioma growth by downregulating PD-L1 expression via the NOD-like receptor/NFKB1/STAT1 axis; Authors - Jing Chen
human	IF	Circ Res; Spatiotemporal ATF3 Expression Determines VSMC Fate in Abdominal Aortic Aneurysm; Authors - Ying Wen
mouse	IF,WB	Cell Commun Signal; Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation; Authors - Xuan Li