NFKB2,p52,p100-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NFKB2,p52,p100-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 15503-1-AP

Synonyme

NFKB2, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1, LYT 10

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  WB analysis using 15503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 15503-1-AP

  WB result of NFKB2,p52,p100-Specific antibody (15503-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and sg-NFKB2 transfected HAP1 cells.

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  IHC staining of human spleen using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15503-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKB2,p52,p100-Specific antibody (15503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 有核膜 (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15503-1-AP bindet in WB, IHC, IF/ICC, ELISA NFKB2,p52,p100-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 52/100 kDa
• GenBank-Zugangsnummer: NM_001077494
• Gene symbol: NFKB2
• Gene ID (NCBI): 4791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind both p52 and p100 isoforms of NFKB2.

Publikationen

Species	Application	Title
human	IHC	Cancers (Basel); Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.; Authors - Ha-Yeon Shin
human	WB,IF	Sci Rep; TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-κB pathway.; Authors - Jun Wang
human	WB	Pharm Biol; Simiao Qingwen Baidu decoction inhibits Epstein-Barr virus-induced B lymphoproliferative disease and lytic viral replication.; Authors - Xianhui Yang
human	WB	Mol Cell Endocrinol; Interleukin-1 increases cyclooxygenase-2 expression and prostaglandin E2 production in human granulosa-lutein cell via nuclear factor kappa B/P65 and extracellular signal-regulated kinase 1/2 signaling pathways; Authors - Shan Wan ; KD Validated
human	WB	Theranostics; Platelet membrane-coated alterbrassicene A nanoparticle inhibits calcification of the aortic valve by suppressing phosphorylation P65 NF-κB; Authors - Bingchuan Geng
human	WB	Oncogene; Monoacylglycerol lipase blockades the senescence-associated secretory phenotype by interfering with NF-κB activation and promotes docetaxel efficacy in prostate cancer; Authors - Jianpeng Yu