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NFKB2,p100 Polyklonaler Antikörper

NFKB2,p100 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 10409-2-AP

Synonyme

NFKB2, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1, LYT 10



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
Erfolgreiche IPK-562-Zellen
Erfolgreiche Detektion in IHChumanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCU2OS-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:12000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10409-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA NFKB2,p100 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NFKB2,p100 fusion protein Ag0662
Vollständiger Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Berechnetes Molekulargewicht 97 kDa
Beobachtetes Molekulargewicht 100-110 kDa
GenBank-ZugangsnummerBC002844
Gene symbol NFKB2
Gene ID (NCBI) 4791
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for NFKB2,p100 antibody 10409-2-APProtokoll herunterladen
IHC protocol for NFKB2,p100 antibody 10409-2-APProtokoll herunterladenl
IF protocol for NFKB2,p100 antibody 10409-2-APProtokoll herunterladen
IP protocol for NFKB2,p100 antibody 10409-2-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nat Commun

A proteomics landscape of circadian clock in mouse liver.

Authors - Yunzhi Wang
humanWB

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
mouseWB

J Hazard Mater

TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2.

Authors - Shuang Li
humanWB

PLoS Pathog

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Authors - Muyu Xu
humanWB,IHC

Biochim Biophys Acta Mol Basis Dis

LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway

Authors - Haifeng Zhang
humanWB

Cell Death Dis

Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.

Authors - Xinhua Xiao
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