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- KD/KO Validated
NFKB2,p100 Polyklonaler Antikörper
NFKB2,p100 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10409-2-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen | 
| Erfolgreiche IP | K-562-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Pankreaskarzinomgewebe,  humanes Leberkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | U2OS-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:20-1:200 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below | 
| WB | See 16 publications below | 
| IHC | See 8 publications below | 
| IF | See 2 publications below | 
Produktinformation
10409-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA NFKB2,p100 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus | 
| In Publikationen genannte Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | NFKB2,p100 fusion protein Ag0662 | 
| Vollständiger Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) | 
| Berechnetes Molekulargewicht | 97 kDa | 
| Beobachtetes Molekulargewicht | 100-110 kDa | 
| GenBank-Zugangsnummer | BC002844 | 
| Gene symbol | NFKB2 | 
| Gene ID (NCBI) | 4791 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for NFKB2,p100 antibody 10409-2-AP | Protokoll herunterladen | 
| IHC protocol for NFKB2,p100 antibody 10409-2-AP | Protokoll herunterladenl | 
| IF protocol for NFKB2,p100 antibody 10409-2-AP | Protokoll herunterladen | 
| IP protocol for NFKB2,p100 antibody 10409-2-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| J Hazard Mater TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2. | ||
| PLoS Pathog SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy. | ||
| Biochim Biophys Acta Mol Basis Dis LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway | ||
| Cell Death Dis Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma. | 







