NFKB2 Monoklonaler Antikörper

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• KD/KO Validated

NFKB2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

6A10E9

Kat-Nr. : 66920-1-PBS

Synonyme

6A10E9, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1, LYT 10

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  WB analysis using 66920-1-Ig (same clone as 66920-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66920-1-Ig (NFKB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

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  WB analysis using 66920-1-Ig (same clone as 66920-1-PBS)

  N/A This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66920-1-Ig (same clone as 66920-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66920-1-PBS bindet in WB, IHC, ELISA NFKB2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NFKB2 fusion protein Ag28543
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC002844
• Gene symbol: NFKB2
• Gene ID (NCBI): 4791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.